普罗亭 普罗亭
质谱流式细胞技术服务
普罗亭提供从定制化的方案、样本处理、抗体标记、检测上机到数据分析的全流程检测服务。
技术原理 技术特点 应用领域 服务流程 样本类型 数据分析 服务优势 服务成果 Q&A
普罗亭
技术原理
质谱流式细胞技术(Mass Cytometry, CyTOF)采用带有金属同位素标签的抗体对细胞进行标记,通过飞行时间质谱分析各细胞上的标签组成,进行细胞表型和功能的深入研究。
技术特点
普罗亭

超高通道 质谱流式具有非常宽的原子量检测范围(80-209 Da),理论上可以同时检测130个通道

普罗亭

超高性价比 单个细胞可同时检测42个Marker,大大节约了实验成本,并减少了实验准备时间

普罗亭

超高分辨率 金属标签替代荧光标签,无串色干扰、无需补偿、背景极低,可深入研究各细胞亚群的功能及细胞亚群间的联系,实现更加精细的细胞分型

普罗亭

超多维度 多样化的数据处理方式,实现对样本的深入数据挖掘

普罗亭
普罗亭
普罗亭
服务流程
普罗亭采用多对一的项目合作模式,针对单个项目建立包含多名专业技术人员的专项团队。
深入了解客户需求,从合同签订到结项报告全流程有效沟通并严格质控。提供更加专业、放心的质谱流式完整解决方案。
普罗亭
样本类型
人源样本包括肝癌、肺癌、胃癌等癌组织和斑块、血管、 皮肤等非癌组织。
小鼠样本包括小肠癌、直肠癌、黑色素瘤等癌组织和脑膜,心脏、骨髓等非癌组织。
涉及实体瘤、血液病、自身免疫病、心血管疾病、呼吸系统疾病等多个领域,每年检测量超10000例。
普罗亭
人源样本
普罗亭
小鼠样本
数据分析
普罗亭生信分析团队的核心科学家博士毕业于美国拥有顶级生物医学工程专业 的佐治亚理工学院,核心算法的系列
软件均为自主研发,拥有正式授权的软件著作权35项。能够为客户提供定制化的数据挖掘方案,满足多样化的数据分析挖掘需求。
普罗亭
普罗亭
普罗亭
普罗亭
普罗亭
普罗亭
普罗亭
普罗亭
heatmap
热图展示经生信算法自动解析的亚群cluster与其marker表达模式
tSNE
降维可视化展示不同cluster、group细胞以及marker表达的空间分布
Frequency bar
不同sample、group的亚群比例叠加图
boxplot
统计学分析出不同group间差异的细胞表型
Correlation
不同cluster、marker间相关性分析
Marker density
展示不同cluster、group间marker的表达分布密度
MST
展示所有细胞亚群及marker的空间相关性分布
Pseudotime
拟时序分析细胞群体发育轨迹
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08
普罗亭服务优势
服务成果
文献125篇,累计IF>1600,平均15,10分以上达50%
  • 合作单位
    发表文章
    发表期刊
    IF
    发表时间
  • 北京协和医院
    PD-1 and TIGIT coexpressing CD8 + CD103 + tissue-resident memory cells in endometrial cancer as potential targets for immunotherapy
    International Immunopharmacology
    5.60
    2023-12
  • 福建医科大学附属协和医院
    Correction to: A modified immune cell infiltration score achieves ideal stratification for CD8+ T cell efficacy and immunotherapy benefit in hepatocellular carcinoma
    Cancer Immunol Immunother.
    5.80
    2023-12
  • 福建医科大学附属协和医院
    TGF-β-p-STAT1-LAIR2 axis has a "self-rescue" role for exhausted CD8+ T cells in hepatocellular carcinoma
    Cell Oncol (Dordr).
    6.60
    2023-12
  • 福建医科大学附属协和医院
    SUMOylation inhibitors activate anti-tumor immunity by reshaping the immune microenvironment in a preclinical model of hepatocellular carcinoma
    Cell Oncol (Dordr).
    6.60
    2023-12
  • 浙江大学
    Downregulation of N4-acetylcytidine modification in myeloid cells attenuates immunotherapy and exacerbates hepatocellular carcinoma progression
    Br J Cancer.
    8.80
    2023-12
  • 解放军总医院第五医学中心
    Characteristics of blood immune cell profile and their correlation with disease progression in patients infected with HIV-1
    BMC Infect Dis.
    3.70
    2023-12
  • 华西口腔医院
    Mass cytometry and transcriptomic profiling reveal PD1 blockade induced alterations in oral carcinogenesis
    Mol Carcinog.
    4.60
    2023-12
  • 浙江大学医学院附属第一人民医院
    TP53/mTORC1-mediated bidirectional regulation of PD-L1 modulates immune evasion in hepatocellular carcinoma
    J Immunother Cancer.
    10.90
    2023-12
  • 广州市第一人民医院
    High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis
    Front Immunol.
    7.30
    2023-12
  • 上海交通大学医学院附属瑞金医院
    High-dimensional single-cell proteomics analysis of esophageal squamous cell carcinoma reveals dynamic alterations of the tumor immune microenvironment after neoadjuvant therapy
    J Immunother Cancer
    10.90
    2023-11
  • 福建医科大学附属协和医院
    Single-cell atlas reveals characteristic changes in intrahepatic HBV-specific leukocytes
    Microbiol Spectr.
    3,70
    2023-11
  • 长海医院
    CTM2-2023-06-1111: Targeting regulatory T-cells in pancreas during acute pancreatitis: Programmed-death 1 blockage as a potential therapeutic for infectious pancreatic necrosis
    Clin Transl Med.
    10.60
    2023-11
  • 浙江大学附属第二医院
    Age-Related Alterations in Peripheral Immune Landscape with Magnified Impact on Post-Stroke Brain
    Research (Wash D C).
    11.00
    2023-11
  • 南京医科大学附属第一医院
    Single-cell RNA-sequencing atlas reveals an FABP1-dependent immunosuppressive environment in hepatocellular carcinoma
    J Immunother Cancer
    10.90
    2023-11
  • 浙江大学附属第一医院
    Characteristics of circulating immune cells in HBV-related acute-on-chronic liver failure following artificial liver treatment
    BMC Immunol
    3.00
    2023-11
  • 广州市第一人民医院
    High-dimensional immune profiling using mass cytometry reveals IL-17A-producing γδT cells as biomarkers in patients with T-cell-activated idiopathic severe aplastic anemia
    Int Immunopharmacol
    5.60
    2023-11
  • 鲁南制药
    Intestinal flora, intestinal metabolism, and intestinal immunity changes in complete Freud's adjuvant-rheumatoid arthritis C57BL/6 mice
    Int Immunopharmacol
    5.60
    2023-10
  • 南京医科大学附属第一医院
    Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma
    Journal of Hepatology
    25.70
    2023-10
  • 复旦大学附属中山医院
    PGAM1 Inhibition Promotes HCC Ferroptosis and Synergizes with Anti-PD-1 Immunotherapy
    Adv Sci (Weinh)
    15.10
    2023-10
  • 上海交通大学医学院附属瑞金医院
    Systemic immune profiling of Omicron-infected subjects inoculated with different doses of inactivated virus vaccine
    Cell
    64.50
    2023-09
  • 上海市第一人民医院
    Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance
    iScience
    5.80
    2023-09
  • 中国人民解放军总医院
    Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice
    Front Endocrinol (Lausanne)
    5.20
    2023-09
  • 复旦大学附属华山医院
    Single-cell mapping reveals several immune subsets associated with liver metastasis of pancreatic ductal adenocarcinoma
    Med.
    17.00
    2023-08
  • 浙江大学医学院附属第一人民医院
    BMI1-induced CD127+KLRG1+ memory T cells enhance the efficacy of liver cancer immunotherapy
    Cancer Letters
    9.70
    2023-08
  • 复旦大学附属中山医院
    A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia
    Nat Commun.
    16.60
    2023-08
  • 南方医科大学南方医院
    Tagering MS4A4A on tumour-associated macrophages restores CD8+T-cell-mediated antitumout immunity
    Gut
    24.50
    2023-07
  • 浙江大学附属第二医院
    Cytokine and chemokine map of peripheral specific immune cell subsets in Parkinson's disease
    NPJ Parkinsons Disease
    8.70
    2023-07
  • 浙江大学
    A gain-of-function variation in PLCG1 causes a new immune dysregulation disease
    J Allergy Clin Immunol
    14,20
    2023-07
  • 复旦大学附属中山医院
    Intratumoral PPT1-positive macrophages determine immunosuppressive contexture and immunotherapy response in hepatocellular carcinoma
    Journal for Immunotherapy of Cancer
    10.90
    2023-06
  • 复旦大学
    Hydroxyphenylpyruvate Dioxygenase Is a Metabolic Immune Checkpoint for UTX-deficient Colorectal Cancer
    Gastroenterology
    29.40
    2023-06
  • 浙江大学医学院附属第一医院
    Single-cell analysis reveals HBV-specific PD-1+CD8+ TRM cells in tumor borders are associated with HBV-related hepatic damage and fibrosis in HCC patients
    Clin Cancer Res
    11.50
    2023-06
  • 福建医科大学附属协和医院
    25-dimethylcelecoxib alleviated NK and T-cell exhaustion in hepatocellular carcinoma via the gastrointestinal microbiota-AMPK-mTOR axis
    J Immunother Cancer
    10.90
    2023-06
  • 浙江大学
    Identification of an IL-1 receptor mutation driving autoinflammation directs IL-1-targeted drug design
    Immunity
    32.40
    2023-06
  • 西南大学药学院医学研究所
    Breaking Spatiotemporal Barriers of Immunogenic Chemotherapy via an Endoplasmic Reticulum Membrane-Assisted Liposomal Drug Delivery
    ACS Nano
    17.10
    2023-05
  • 中国科学院北京基因组研究所
    Epigenetic reprogramming of RUNX3 reinforces CD8+T-cell function and improves the clinical response to immunotherapy
    Mol Cancer
    37.30
    2023-05
  • 浙江大学医学院附属第一医院
    Effects of 3-HAA on HCC by Regulating the HeterogeneousMacrophages—A scRNA-Seq Analysis
    Adv Sci (Weinh)
    15.10
    2023-04
  • 中国医学科学院血液病研究所
    Single-cell analysis highlights a population of Th17-polarized CD4 + naïve T cells showing IL6 / JAK3 / STAT3 activation in pediatric severe aplastic anemia
    J Autoimmun.
    12.80
    2023-03
  • 深圳大学医学院过敏与免疫学研究所
    Multidimensional Analysis of Lung Lymph Nodes in a Mouse Model of Allergic Lung Inflammation following PM2.5 and Indeno[1,2,3- cd ]pyrene Exposure
    Environ Health Perspect.
    10.40
    2023-03
  • 浙江大学附属第二医院
    Single-cell Profiling of Tumor Immune Microenvironment Reveals Immune Irresponsiveness in Gastric Signet-ring Cell Carcinoma
    Gastroenterology
    29.40
    2023-03
  • 鲁南制药
    Shouhui Tongbian Capsules induce regression of inflammation to improve intestinal barrier in mice with constipation by targeted binding to Prkaa1: With no obvious toxicity
    Biomedicine & Pharmacotherapy
    7.50
    2023-02
  • 复旦大学附属中山医院
    Disruption of SLFN11 deficiency-induced CCL2 signaling and macrophage M2 polarization potentiates anti-PD-1 therapy efficacy in hepatocellular carcinoma
    Gastroenterology
    29.40
    2023-02
  • 中国医学科学院北京协和医学院微循环研究所
    Mass Cytometry Reveals the Imbalanced Immune State in the Peripheral Blood of Patients with Essential Hypertension
    Cardiovasc Ther.
    3.10
    2023-02
  • 浙江大学附属儿童医院
    Comprehensive mapping of B lymphocyte immune dysfunction in idiopathic nephrotic syndrome children
    J Clin Invest.
    10.60
    2023-02
  • 中国人民解放军总医院
    Decitabine-priming increases anti-PD-1 antitumor efficacy by promoting CD8+ progenitor exhausted T-cell expansion in tumor models
    J Clin Invest.
    8.00
    2023-02
  • 浙江大学附属第二医院
    Secondary myeloid neoplasms after CD19 CAR T therapy in patients with refractory/relapsed B-cell lymphoma: Case series and review of literature
    Frontiers in Immunology
    8.786
    2023-01
  • 浙江大学附属第一医院
    An Ionic Liquid Ablation Agent for Local Ablation and Immune Activation in Pancreatic Cance
    Adv Sci (Weinh)
    17.521
    2023-01
  • 浙江省人民医院
    Depiction of immune heterogeneity of peripheral blood from patients with type II diabetic nephropathy based on mass cytometry
    Front Endocrinol (Lausanne)
    6.055
    2023-01
  • 北京大学深圳医院
    Phenotypic and functional alteration of CD45+ immune cells in the decidua of preeclampsia patients analyzed by mass cytometry (CyTOF)
    Frontiers in Immunolog
    8.786
    2023-01
  • 杭州市第一人民医院
    SULT2B1-CS-DOCK2 axis regulates effector T cell exhaustion in hepatocellular carcinoma microenvironment
    Hepatology.
    17.298
    2023-01
  • 广州中医药大学附属第二医院(广东省中医院)
    Single-Cell Analysis in Blood Reveals Distinct Immune Cell Profiles in Gouty Arthritis
    J Immunol.
    5.426
    2023-01
  • 南京医科大学附属第一医院
    Macrophage GSK3β-deficiency inhibits the progression of hepatocellular carcinoma and enhances the sensitivity of anti-PD1 immunotherapy
    Journal for ImmunoTherapy of Cancer
    12.469
    2022-12
  • 重庆医科大学附属第一医院
    Single-cell mapping reveals dysregulation of immune cell populations and VISTA+ monocytes in myasthenia gravis
    Clin Immunol.
    10.19
    2022-12
  • 香港中文大学理工学院深圳综合科学与技术研究院
    High-dimensional single-cell cartography tracking of immune cells subpopulation of mice peripheral blood treated with gold nanorods and black phosphorus nanosheets
    Nano Today
    18.962
    2022-11
  • 浙江大学附属第二医院
    The epidermal immune microenvironment plays a dominant role in psoriasis development, as revealed by mass cytometry
    Cell Mol Immunol.
    22.096
    2022-11
  • 浙江大学
    The immune landscape of human thymic epithelial tumors
    Nat Commun.
    17.694
    2022-10
  • 青岛大学附属青岛市立医院
    The Polygenic Map of Keloid Fibroblasts Reveals Fibrosis-Associated Gene Alterations in Inflammation and Immune Responses
    Frontiers in Immunolog
    8.786
    2022-10
  • 浙江大学附属第一医院
    Pyroptosis Remodeling Tumor Microenvironment to Enhance Pancreatic Cancer Immunotherapy Driven by Membrane Anchoring Photosensitizer
    Adv Sci (Weinh)
    17.521
    2022-10
  • 南京医科大学附属第一医院
    Inhibition of APOC1 promotes the transformation of M2 into M1 macrophages via the ferroptosis pathway and enhances anti-PD1 immunotherapy in hepatocellular carcinoma based on single-cell RNA sequencing
    Redox Biol
    10.787
    2022-10
  • 浙江大学附属第一医院
    Immune-infiltrating signature based classification reveals CD103+CD39+ T cells associate with colorectal cancer prognosis and response to immunotherapy
    Front Immunol.
    8.786
    2022-10
  • 浙江大学附属第一医院
    Comprehensive immune cell analysis of human menstrual-blood-derived stem cells therapy to concanavalin A hepatitis
    Front Immunol.
    8.786
    2022-09
  • 杭州市第一人民医院
    Prediction of tumor recurrence by distinct immunoprofiles in liver transplant patients based on mass cytometry
    Am J Cancer Res .
    5.942
    2022-09
  • 北京协和医院
    Single-cell profiling identifies mechanisms of inflammatory heterogeneity in chronic rhinosinusitis
    Nat Immunol
    31.250
    2022-09
  • 浙江大学附属第一医院
    Mass cytometry-based peripheral blood analysis as a novel tool for early detection of solid tumours: a multicentre study
    Gut
    31.793
    2022-09
  • 浙江大学附属第二医院
    High-dimensional Single-cell Analysis Delineates Peripheral Immune Signature of Coronary Atherosclerosis in Human Blood
    Theranostics
    11.600
    2022-09
  • 郑州大学第二附属医院
    Case report: Single-cell mapping of peripheral blood mononuclear cells from a patient with both Crohn's disease and isolated congenital asplenia
    Front Immunol.
    8.786
    2022-09
  • 北京协和医院
    Single-Cell Profiling of the Immune Atlas of Tumor-Infiltrating Lymphocytes in Endometrial Carcinoma
    Cancers (Basel).
    6.575
    2022-09
  • 南京医科大学附属第一医院
    Inhibition of APOE potentiates immune checkpoint therapy for cancer
    Int J Biol Sci
    10.75
    2022-08
  • 复旦大学附属中山医院
    Peripheral naïve CD8+ T cells as a predictive biomarker of response to lenvatinib plus an anti-PD-1 antibody in advanced hepatocellular carcinoma: a biomarker study
    Cancer Commun(Lond)
    15.283
    2022-08
  • 南京医科大学附属第一医院
    Over-Expression of GUSB Leads to Primary Resistance of Anti-PD1 Therapy in Hepatocellular Carcinoma
    Front Immunol.
    8.786
    2022-06
  • 南京医科大学附属第一医院
    MGP promotes CD8+ T cell exhaustion by activating the NF-κB pathway leading to liver metastasis of colorectal cancer
    Int J Biol Sci
    10.75
    2022-03
  • 浙江大学附属第一医院
    Engineered a dual-targeting biomimetic nanomedicine for pancreatic cancer chemoimmunotherapy
    Journal of Nanobiotechnology
    9.429
    2022-02
  • 浙江大学
    Applications of Single-Cell Omics in Tumor Immunology
    Frontiers in Immunolog
    8.786
    2021-06
  • 浙江大学附属第一医院
    Decoding the single-cell landscape and intercellular crosstalk in the transplanted liver: a 4-dimension mouse model
    Transplantation
    5.385
    2022-07
  • 浙江大学
    Metal-Chelatable Porphyrinic Frameworks for Single-Cell Multiplexing with Mass Cytometry
    Angew Chem Int Ed Engl.
    16.823
    2022-07
  • 广州呼吸健康研究院
    Immunoassay and mass cytometry revealed immunological profiles induced by inactivated BBIBP COVID-19 vaccine
    J Med Virol.
    20.693
    2022-07
  • 武汉大学口腔医学院
    Single-cell analysis reveals immune cellular components in odontogenic keratocysts
    Oral Dis
    4.068
    2022-06
  • 南京大学附属鼓楼医院
    Mass cytometry reveals immune atlas of urothelial carcinoma
    BMC cancer
    4.638
    2022-06
  • 浙江大学附属第一医院
    Dynamic Immune Landscape and VZV-Specific T Cell Responses in Patients With Herpes Zoster and Postherpetic Neuralgia
    Frontiers in Immunolog
    8.786
    2022-06
  • 山东大学
    Targeting tissue-resident memory CD8 + T cells in the kidney is a potential therapeutic strategy to ameliorate podocyte injury and glomerulosclerosis
    Molecular therapy
    12.910
    2022-05
  • 华西医院
    Integrating single-cell RNA sequencing with spatial transcriptomics reveals immune landscape for interstitial cystitis
    Signal Transduct Target Ther.
    38.104
    2022-05
  • 复旦大学肿瘤医院
    Hypoxia-reprogrammed regulatory group 2 innate lymphoid cells promote immunosuppression in pancreatic cancer
    eBioMedicine
    11.205
    2022-04
  • 复旦大学附属中山医院
    Interferon-α potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment
    Cancer Discovery
    38.272
    2022-04
  • 北京大学人民医院
    Lung adenocarcinoma manifesting as subsolid nodule potentially represents tumor in the equilibrium phase of immunoediting
    Immunology
    7.251
    2022-03
  • 浙江大学附属第一医院
    Dynamic profiling of immune microenvironment during pancreatic cancer development suggests early intervention and combination strategy of immunotherapy
    eBioMedicine
    11.205
    2022-03
  • 浙江大学附属第一医院
    Combination cancer immunotherapy targeting TNFR2 and PD-1/PD-L1 signaling reduces immunosuppressive effects in the microenvironment of pancreatic tumors
    Journal for ImmunoTherapy of Cancer
    12.469
    2022-03
  • 浙江大学附属邵逸夫医院
    Systemic and single cell level responses to 1 nm size biomaterials demonstrate distinct biological effects revealed by multi-omics atlas
    Bioactive materials
    16.874
    2022-03
  • 复旦大学附属中山医院
    PKCα/ZFP64/CSF1 axis resets the tumor microenvironment and fuels anti-PD1 resistance in hepatocellular carcinoma
    Journal of hepatology
    30.083
    2022-02
  • 浙江大学附属第二医院
    Specific immune status in Parkinson's disease at different ages of onset
    npj Parkinson's Disease
    9.304
    2022-01
  • 浙江大学附属第一医院
    Single-Cell immune signature for detecting early-stage HCC and early assessing anti-PD-1 immunotherapy efficacy
    Journal for ImmunoTherapy of Cancer
    12.469
    2022-01
  • 中南大学湘雅医院
    Engineering an enthesis-like graft for rotator cuff repair: An approach to fabricate highly biomimetic scaffold capable of zone-specifically releasing stem cell differentiation inducers
    Bioactive materials
    16.874
    2022-01
  • 深圳市第三人民医院
    Discovery of CD3+CD19+ cells, a novel lymphocyte subset with a potential role in human immunodeficiency virus-Mycobacterium tuberculosis coinfection, using mass cytometry
    Clinical and translational medicine
    8.554
    2021-12
  • 浙江大学
    Rational administration sequencing of immunochemotherapy elicits powerful anti-tumor effect
    Journal of Controlled Release
    11.467
    2021-12
  • 长海医院
    An Immune Atlas of Nephrolithiasis: Single-Cell Mass Cytometryon SIRT3 Knockout and Calcium Oxalate-Induced Renal Injury
    Journal of Immunology Research
    4.493
    2021-11
  • 复旦大学附属中山医院
    A cellular census of human peripheral immune cells identifies novel cell states in lung diseases
    Clinical and translational medicine
    8.554
    2021-11
  • 南方医科大学珠江医院
    Single-Cell RNA Sequencing Reveals the Heterogeneity of Tumor-Associated Macrophage in Non-Small Cell Lung Cancer and Differences Between Sexes
    Frontiers in Immunolog
    8.786
    2021-11
  • 浙江大学
    Pro-Inflammatory Signature in Decidua of Recurrent Pregnancy Loss Regardless of Embryonic Chromosomal Abnormalities
    Frontiers in Immunolog
    8.786
    2021-11
  • 复旦大学附属瑞金医院
    Distinct immune signatures discriminate between asymptomatic and presymptomatic SARS-CoV-2 pos subjects
    Cell Research
    46.297
    2021-11
  • 河南省肿瘤医院
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    Cancer Letters
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    EBioMedicine
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    Cell Prolification
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    Frontiers in Immunolog
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    Hepatology
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    Theranostics
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    Cell Death & Disease
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北京生命科学研究所北京生命科学研究所
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Q&A
CyTOF和流式的区别?
简单来说CyTOF就是高通量流式,采用金属同位素标签替代传统流式的荧光标签来标记细胞,并通过飞行时间质谱来检测每个细胞上金属标签的精确含量。和传统流式相比,CyTOF不会存在“串色”的问题,不需要补偿计算,因此可以同时检测更多种蛋白,普罗亭目前能够同时测42个marker,而流式很难超过20个marker,所以CyTOF可能发现流式难以观察到的一些价值信息。
CyTOF能检测什么样本?
组织和外周血都可以测,也可以直接提供细胞悬液或者细胞系。
CyTOF的样本要求有什么?
有细胞数量的要求,血样的细胞数量要达到3×10^6,而组织,消化后的细胞数量要达到1×10^6。此外,新鲜样本需要在取样后48小时内寄送至普罗亭实验室。
样本如何寄送?
普罗亭有标准的样本保存和运输手册。您只需按照我们提供的样本保存方法和寄送方式,在取样后48小时内寄送至普罗亭实验室即可。
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